您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 产品库 > Icaritin
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
Icaritin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Icaritin图片
CAS NO:118525-40-9
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
50mg电议
100mg电议

产品名称
脱水淫羊藿素
Anhydroicaritin
产品介绍
Icaritin (Anhydroicaritin) 是淫羊藿中的异戊二烯类黄酮衍生物,有效抑制 K562 细胞 (IC50为 8 μM) 和原代 CML 细胞 (对 CML-CP 的IC50值为 13.4 μM,对 CML-BC 的IC50值为 18 μM) 的增殖。Icaritin 可以调节MAPK/ERK/JNKJAK2/STAT3/AKT信号传导,并具有增强成骨的作用。
生物活性

Icaritin (Anhydroicaritin) is a prenylflavonoid derivative fromEpimedium brevicornuMaxim. and potently inhibits proliferation of K562 cells (IC50of 8 μM) and primary CML cells (IC50of 13.4 μM for CML-CP and 18 μM for CML-BC). Icaritin can regulateMAPK/ERK/JNKandJAK2/STAT3 /AKTsignalings, also enhances osteogenesis[1][2][3.

体外研究
(In Vitro)

Icaritin (4-64 μM; 48 hours; K562, imatinib-resistant cells and primary CML cells) treatment inhibits proliferation of K562, imatinib-resistant cells and primary CML cells[1].
Icaritin (0-64 μM; 48 hours; K562 and primary cells) treatment induces K562 or primary cells apoptosis in an concentration dependent manner[1].
Icaritin (32 μM; K562 cells) treatment increases cell population in the sub-G1 phase in K562 cells[1].
Icaritin (0-64 μM; 48 hours; K562 cells) treatment inhibits MAPK/ERK/JNK downstream signaling and diminishes Jak2/Stat3/Akt expression. Icaritin treatment also significantly inhibits Bcl-2 protein expression and up-regulated Bax protein expression in K562 with a dose-dependent manner accompanied by the cleavage activation of caspase-3 or caspase-9, and a down-regulated expression of Apaf-1[1].

Cell Proliferation Assay[1]

Cell Line:K562, imatinib-resistant cells and primary CML cells
Concentration:4 μM, 8 μM, 16 μM, 32 μM and 64 μM
Incubation Time:48 hours
Result:Inhibited cell proliferation.

Apoptosis Analysis[1]

Cell Line:K562 or primary cells
Concentration:0 μM, 4 μM, 8 μM, 16 μM, 32 μM and 64 μM
Incubation Time:48 hours
Result:Induced K562 or primary cells apoptosis.

Cell Cycle Analysis[1]

Cell Line:K562 cells
Concentration:32 μM
Incubation Time:
Result:Cell population in the sub-G1 phase was increased.

Western Blot Analysis[1]

Cell Line:K562 cells
Concentration:0 μM, 4 μM, 8 μM, 16 μM, 32 μM and 64 μM
Incubation Time:48 hours
Result:Inhibited MAPK/ERK/JNK downstream signaling and diminishes Jak2/Stat3/Akt expression.
体内研究
(In Vivo)

Icaritin (4-8 mg/kg; intraperitoneal injection; daily; for 10 weeks; female NOD-SCID nude mice) treatment could prolong lifespan of NOD-SCID nude mice inoculated with K562 cells without suppression of bone marrow in mouse leukemia model[1].

Animal Model:Female NOD-SCID nude mice (6-8 weeks old) with K562 cells[1]
Dosage:4 mg/kg and 8 mg/kg
Administration:Intraperitoneal injection; daily; for 10 weeks
Result:Could prolong lifespan of NOD-SCID nude mice inoculated with K562 cells without suppression of bone marrow.
Clinical Trial
分子量

368.38

性状

Solid

Formula

C21H20O6

CAS 号

118525-40-9

中文名称

去水淫羊藿黄素;三七淫羊藿素

结构分类
  • Flavonoids
  • Flavonols
  • Phenols
  • Polyphenols
来源
  • Plants
  • Berberidaceae
  • Epimedium brevicornuMaxim.
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 15.62 mg/mL(42.40 mM;Need ultrasonic)

H2O : 1.2 mg/mL(3.26 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.7146 mL13.5729 mL27.1459 mL
5 mM0.5429 mL2.7146 mL5.4292 mL
10 mM0.2715 mL1.3573 mL2.7146 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 1.56 mg/mL (4.23 mM); Clear solution

    此方案可获得 ≥ 1.56 mg/mL (4.23 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 15.6 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 50%PEG300   50% saline

    Solubility: 1.51 mg/mL (4.10 mM); Suspended solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。