CWP232228

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CWP232228

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

1144044-02-9

包装与价格：

包装	价格(元)
5mg	电议
10mg	电议
50mg	电议
100mg	电议

产品介绍

CWP232228 是一种高效的选择性Wnt/β-catenin信号通路抑制剂，在细胞核中，拮抗 β-catenin 与 T 细胞因子 (TCF) 结合。CWP232228 通过抑制乳腺癌和肝癌干细胞 (CSCs) 的生长来抑制肿瘤的形成和转移而无毒性。

生物活性

CWP232228, a highly potent selectiveWnt/β-cateninsignaling inhibitor, antagonizes binding ofβ-cateninto T-cell factor (TCF) in the nucleus. CWP232228 suppresses tumor formation and metastasis without toxicity through the inhibition of the growth of breast and livercancer stem cells(CSCs)^[1].

IC₅₀& Target

Wnt/β-catenin^[1]

体外研究
(In Vitro)

CWP232228 (0.01-100 μM; 48 hours) inhibits cell proliferation with IC₅₀values are 2 and 0.8 μM in mouse (4T1) and human (MDA-MB-435) breast cancer cell lines, respectively^[1].
CWP232228 (0.01-10 μM; 48 hours) inhibits cell proliferation with IC₅₀s of 2.566, 2.630, and 2.596 μM in Hep3B, Huh7 and HepG2 cells, respectively^[2].

Cell Proliferation Assay^[1]

Cell Line:	Mouse (4T1) and human (MDA-MB-435) breast cancer cell lines
Concentration:	0.01, 0.1, 1, 10, 100 μM
Incubation Time:	48 hours
Result:	IC₅₀s were 2 and 0.8 μM for 4T1 and MDA-MB-435 cell lines, respectively.

Cell Proliferation Assay^[2]

Cell Line:	Hepatocellular carcinoma cell lines HepG2, Huh7, and Hep3B
Concentration:	0.01, 0.1, 0.5, 1, 5, 10 μM
Incubation Time:	48 hours
Result:	IC₅₀s were 2.566, 2.630, and 2.596 μM for Hep3B, Huh7 and HepG2 cells, respectively.

体内研究
(In Vivo)

CWP232228 (100 mg/kg, administered i.p.; daily; 21days for mice bearing 4T1 cell tumors; 60 days for mice bearing MDA-MB-435 cell tumors) results in a significant reduction in tumor volume^[1].

Animal Model:	7-week-old female Balb/c and NOD/SCID mice bearing 4T1 or MDA-MB-435 cell tumors^[1]
Dosage:	100 mg/kg
Administration:	Administered i.p.; daily; 21days for mice bearing 4T1 cell tumors, 60 days for mice bearing MDA-MB-435 cell tumors
Result:	Treatment resulted in a significant reduction in tumor volume.

分子量

717.62

性状

Solid

Formula

C₃₃H₃₄N₇Na₂O₇P

CAS 号

1144044-02-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

H₂O : 62.5 mg/mL(87.09 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	1.3935 mL	6.9675 mL	13.9350 mL
5 mM	0.2787 mL	1.3935 mL	2.7870 mL
10 mM	0.1393 mL	0.6967 mL	1.3935 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 50 mg/mL (69.67 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。