| 包装 | 价格(元) |
| 1mg | 电议 |
| 5mg | 电议 |
| 10mg | 电议 |
Cell lines | RAW264.7 cells |
Preparation method | The solubility of this compound in DMSO is >25 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
Reacting condition | 48 h, 0.5 μg/mL |
Applications | In RAW264.7 cells, tunicamycin significantly reduced LPS-induced nitrite release/production and attenuated the expression of mRNAs and hence proteins of COX-2 and iNOS. Tunicamycin at a concentration of 0.5 μg/mL did not have any effect on cell survival/proliferation, but at 48 h tunicamycin provided protection against activation-induced macrophage cell death. In a concentration-dependent manner, tunicamycin reduced COX-2 and iNOS protein expression in response to LPS and induced a concurrent increase in 78-kDa glucose-regulated protein (GRP78), an ER (endoplasmic reticulum) chaperone. |
Animal models | C57BL/6J Nrf2 (+/+; wildtype) and C57BL/6J/Nrf2(-/-; knockout) mice |
Dosage form | Oral gavage and only once for 3 h with 2 mg/kg tunicamycin (dissolved in 50% PEG 400 aqueous solution). |
Application | In the small intestine of wild-type mice, expression levels of 1291 probes were elevated or of 1370 probes were suppressed >2 fold by tunicamycin. In the small intestine of Nrf2(-/-) mice, tunicamycin inhibited 2024 probes and induced 3471 probes by >2 fold. Compared with results of small intestine samples, in wild-type mice liver, less well-defined genes were either suppressed (943) or elevated (750) >2 fold by tunicamycin; whereas in Nrf2(-/-) mice liver, 3170 genes were inhibited or 39 well-defined genes were induced. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
| 文献引用 | |
| 产品描述 | Tunicamycin (TCM or TM) [1] [2] is an antibiotic. It can block the reaction between UDP-N-acetylglucosamine and dolichol phosphate in the first step of glycoprotein synthesis and thus inhibit the synthesis of all N-linked glycoproteins, finally cause endoplasmic reticulum (ER) stress [3]. In Bacillus subtilis cells, the IC50 for TCM to inhibit the formation of dolichyl pyrophosphoryl N-acetylglucosamine (Dol-p-p-GlcNAc) is 0.03 μg/ml [2]. The ER stress response is a potent, evolutionarily conserved response to cellular metabolic stress and misfolded proteins. ER stress is induced by disruption of ER functions, such as transport to the Golgi complex or protein glycosylation, or by disturbances in the ER lumen environment, such as redox status or altered calcium homeostasis [3]. In RAW264.7 cells, tunicamycin significantly reduced LPS-induced nitrite release/production and attenuated the expression of mRNAs and hence proteins of COX-2 and iNOS. In addition, tunicamycin at a concentration of 0.5 μg/ml did not have any effect on cell survival/proliferation, but at 48h tunicamycin provided protection against activation-induced macrophage cell death. In a concentration-dependent manner, tunicamycin reduced COX-2 and iNOS protein expressions in response to LPS and induced a concurrent increase in 78-kDa glucose-regulated protein (GRP78), an ER chaperone [3]. In the small intestine of wild-type mice, tunicamycin elevated expression levels of suppressed 1370 probes and 1291 probes by >2 fold. In the small intestine of Nrf 2 (-/-) mice, tunicamycin inhibited 2024 probes and induced 3471 probes by >2 fold. Compared with results of small intestine samples, in wild-type mice liver, less well-defined genes were either suppressed (943) or elevated (750) >2 fold by tunicamycin; whereas in Nrf2 (-/-) mice liver, 3170 genes were inhibited or 39 well-defined genes were induced [1]. Reference: |
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