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Piperine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Piperine图片
CAS NO:94-62-2
规格:≥98%
包装与价格:
包装价格(元)
1g电议
5g电议
10g电议
25g电议
50g电议
100g电议

产品介绍
理化性质和储存条件
FormulaC17H19NO3
CAS No.94-62-2
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 57 mg/mL (199.8 mM)
Water: <1 mg/mL
Ethanol: 57 mg/mL (199.8 mM)
Solubility (In vivo)O=C(N1CCCCC1)/C=C/C=C/C2=CC=C(OCO3)C3=C2
SynonymsPiperine; 1-Piperoylpiperidine; Bioperine; 1-Piperoyl piperidine
实验参考方法
In Vitro

In vitro activity: Piperine (1-Piperoylpiperidine) is the alkaloid responsible for the pungency of black pepper and long pepper, along with chavicine (an isomer of piperine). It has also been used in some forms of traditional medicine and as an insecticide. Piperine has been found to inhibit human CYP3A4 and P-glycoprotein, enzymes important for the metabolism and transport of xenobiotics and metabolites. Piperine is found to be cytotoxic towards DLA and EAC cells at a concentration of 250 μ g/ml.


Cell Assay: Standard solution is prepared by dissolving 10 mg of piperine in 100 mL of methanol. The MTT assay is carried out to measure cell viability. Ten thousand cells in 100 μL of DMEM media are seeded in the wells of a 96-well plate. After 24 h, existing media is removed and 100 μL of various concentrations of piperine (20–100 μg/mL) are added and incubated for 48 h at 37 °C in a CO2 incubator. Control cells are supplemented with 0.05 % DMSO vehicle. At the 48th hour of incubation, MTT (10 μL of 5 mg/mL) is added to the plate. The contents of the plate are pipetted out carefully, the formazan crystals formed are dissolved in 100 μL of DMSO, and the absorbance is measured at 550 nm in a microplate reader.

In VivoPiperine (1.14 mg/dose/animal) could inhibit the solid tumor development in mice induced with DLA cells and increase the life span of mice bearing Ehrlich ascites carcinoma tumor. LD50: Mice 15.1mg/kg (i.v.), 43mg/kg (i.p.), 200mg/kg (s.c.), 330mg/kg (i.g.); Rats 33.5mg/kg (i.p.), 514mg/kg (i.g.)
Animal modelMice and rats
Formulation & DosageMice 15.1mg/kg (i.v.), 43mg/kg (i.p.), 200mg/kg (s.c.), 330mg/kg (i.g.); Rats 33.5mg/kg (i.p.), 514mg/kg (i.g.)
References

J Pharmacol Exp Ther. 2002 Aug;302(2):645-50; J Ethnopharmacol. 2004 Feb;90(2-3):339-46; Toxicol Lett. 1983 May;16(3-4):351-9.