GTP酶是GTP/GDP结合蛋白。被细胞表面受体(GPCR)激活后, 它能调节下游效应器的功能。它可以结合GTP、水解GTP，因此在激活的GTP结合状态和非激活的GDP结合状态之间切换。它具有内在的GTP酶活性，可将GTP水解为GDP，从而使G蛋白失活。我们精心筛选的小鼠单克隆抗体能捕捉到与GTP结合的具有活性的GTP酶。它们已被用于通过免疫组织化学监测细胞和组织中 G 蛋白的活化和分布。
| 描述: Anti-Rheb-GTP Monoclonal Antibody |
| 背景介绍: Small GTPases are a super-family of cellular signaling regulators. Rheb is a member of the Ras-superfamily GTPases. Rheb has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related (ATR). |
| 免疫原: Recombinant full-length protein of active Rheb. |
| 经过测试的应用: IP, IHC,IF |
推荐稀释比:
IP: 1 μg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250 |
| 种属反应性: Anti-Rheb-GTP monoclonal antibody recognizes active Rheb of vertebrates. |
| 储存条件: Store at -20°C. Avoid freeze/thaw cycles |
免疫沉淀/蛋白质印迹: 
Rheb activation assay. Purified Rheb proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Rheb-GTP monoclonal antibody (Cat. No. 26910). Immunoblot was with an anti-Rheb rabbit polyclonal antibody (Cat. No. 21098). |
