质粒名称:pMRX-IP-GFP-LC3-RFP-LC3ΔG
质粒编号:84572
质粒备注:Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic flux
载体名称:pMRX-IP
载体大小:6100
完整质粒大小:8300
载体类型:哺乳动物表达,逆转录
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:未知
插入基因:microtubule-associated protein 1 light chain 3 beta
基因别名:Map1lc3b
基因别名:LC3
基因别名:LC3B
物种来源:R. norvegicus (rat)
插入大小:2200
基因名称:Map1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
标签/融合蛋白:· EGFP (N terminal on insert)
· mRFP1 (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:Bgl II (unknown if destroyed)
3'克隆位点:Bam HI (unknown if destroyed)
5'测序引物:CGACCACTACCAGCAGAACA
3'测序引物:AAAAGACGGCAATATGGTGG
质粒图谱: