质粒名称:pBRPyCAG-Mettl3-dsRed-IRES-puro
质粒编号:61516
质粒备注:Constitutive expression of murine wild-type Mettl3
载体名称:pBRPy
完整质粒大小:9810
载体类型:哺乳动物表达
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:Mettl3
物种来源:M. musculus (mouse)
插入大小:1743
基因名称:Mettl3 (a.k.a. 2310024F18Rik, M6A, Spo8)
启动子:CAGG
克隆方法:限制性内切酶
5'克隆位点:Xho (destroyed during cloning)
3'克隆位点:Not (not destroyed)
5'测序引物:GGGGACGGCTGCCTTCGGGG
3'测序引物:ACAGGATGTCCCAGGCGAAG
质粒图谱: