质粒名称:p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A
质粒编号:83485
质粒备注:Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).
载体名称:p2CT
载体大小:5960
完整质粒大小:9437
载体类型:Bacterial Expression, CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:Lbu_C2c2_R472A_H477A_R1048A_H1053A
基因别名:LbuCas13a
物种来源:Leptotrichia buccalis
基因突变:R472A, H477A, R1048A, H1053A
插入大小:3477
克隆方法:Ligation Independent Cloning
5'测序引物:MBP forward
质粒图谱: