质粒名称:lentiMPH v2
质粒编号:89308
质粒备注:lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.
载体名称:plenti
载体类型:哺乳动物表达,慢病毒
筛选标记:潮霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Stbl3
高拷贝/低拷贝:高拷贝
插入基因:MS2-P65-HSF1_2A_Hygro
物种来源:H. sapiens (human), Synthetic
基因突变:N55K in MS2
插入大小:2526
基因名称:RELA (a.k.a. AIF3BL3, CMCU, NFKB3, p65)
启动子:EF1a
克隆方法:限制性内切酶
5'克隆位点:EcoRI (not destroyed)
3'克隆位点:BsiWI (not destroyed)
5'测序引物:cacatagcgtaaaaggagcaacatag
3'测序引物:GTTTGGATCTTGGTTCATTCTCAAGCCTCAG
质粒图谱: