质粒名称:pwtCas9-bacteria
质粒编号:44250
质粒备注:aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown
载体名称:pUC19
完整质粒大小:6932
载体类型:Bacterial Expression, CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Top10
高拷贝/低拷贝:高拷贝
插入基因:wild-type Cas9
物种来源:S .pyogenes
插入大小:4107
启动子:pLtetO-1
克隆方法:限制性内切酶
5'克隆位点:BglII (not destroyed)
3'克隆位点:XhoI (not destroyed)
5'测序引物:agaaaagaattcaaaAGATCTaaagaggagaaaGGATCTATGGATAAGAAATACTCAATAGG
3'测序引物:ggagatccttactcgagttaGTCACCTCCTAGCTGACTCAAATCAAT
质粒图谱: