质粒名称:pKAR1/Pur
质粒编号:23105
质粒备注:(Empty Backbone) Allows expression of shRNA from U6 promoter and a rescue cDNA from a doxycyclin inducible promoter in the same plasmid. Contains puromycin resistance gene
载体名称:pUHD-P3
载体大小:5669
载体类型:Mammalian Expression, RNAi
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:None
启动子:TRE for cDNA; U6 for shRNA
克隆方法:限制性内切酶
5'克隆位点:see paper (not destroyed)
3'克隆位点:see paper (not destroyed)
5'测序引物:LNCX
3'测序引物:EBV-rev