质粒名称:pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)
质粒编号:140003
质粒备注:CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
载体名称:pCMV_ABEmax_P2A_GFP
载体类型:Mammalian Expression, CRISPR ; in vitro transcription; T7 promoter
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
基因别名:RTW5025
物种来源:Synthetic
基因突变:nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R
启动子:CMV and T7
克隆方法:Gibson Cloning
5'测序引物:oBK6928-CCAAGTCTCCACCCCATTGACG
3'测序引物:oBK219-GGGAGTGGCACCTTCCAGGGTC
质粒图谱: