质粒名称:pcDNA-dCas9-p300 Core
质粒编号:61357
质粒备注:encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
载体名称:pcDNA3.1
完整质粒大小:11475
载体类型:Mammalian Expression, CRISPR, Synthetic Biology
筛选标记:新霉素(G418)
原核抗性:氨苄青霉素,100μg/mL
培养温度:30℃
克隆菌株:Stbl3
高拷贝/低拷贝:低拷贝
插入基因:S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300)
物种来源:H. sapiens (human), Synthetic; S. pyogenes
基因突变:D10A; H840A in S.pyogenes Cas9
插入大小:6138
基因名称:EP300 (a.k.a. KAT3B, MKHK2, RSTS2, p300)
启动子:CMV
标签/融合蛋白:· Flag (N terminal on insert)
· SV40 NLS (N terminal on insert)
· SV40 NLS (C terminal on insert)
· HA (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:AscI (not destroyed)
3'克隆位点:PmeI (not destroyed)
5'测序引物:CMV F
3'测序引物:Bgh R
质粒图谱: