质粒名称:T7-VEE-GFP
质粒编号:58977
质粒备注:expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
载体名称:T7-VEE-IRES-puro (Addgene plasmid 58970)
载体类型:Mammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:EGFP
物种来源:Aequorea victoria
启动子:26S subgenomic promoter
克隆方法:限制性内切酶
5'克隆位点:EcoRI (not destroyed)
3'克隆位点:NotI (not destroyed)
5'测序引物:n/a
3'测序引物:pCDH-rev (GCATTCCTTTGGCGAGAG)
质粒图谱: