质粒名称:pYIC
质粒编号:18673
质粒备注:(Empty Backbone)
载体名称:pIRES2-EGFP
载体大小:5300
载体类型:哺乳动物表达
筛选标记:新霉素(G418)
原核抗性:卡那霉素,50μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:Bicistronic fluorescent reporter gene with cap-dependent EYFP and IRES-dependent ECFP translation under the control of CMV promo
物种来源:H. sapiens (human); Aequorea victoria; synthetic sequence.
插入大小:895
标签/融合蛋白:· 3Myc-EYFP-HA-His6 (N terminal on insert)
· ECFP-HA-His6 (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:SacI and BstXI (not destroyed)
3'克隆位点:Eco RI and Not I (not destroyed)
5'测序引物:CMV-F
质粒图谱: