质粒名称:pUC119-gRNA
质粒编号:52255
质粒备注:Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.
载体名称:pUC119
载体大小:3162
载体类型:CRISPR ; Plant expression
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:guide RNA targeting AtPDS3
基因别名:PDS3
基因别名:phytoene desaturase 3
物种来源:A. thaliana (mustard weed)
基因名称:PDS3 (a.k.a. AT4G14210, DL3145C, FCAALL.28, PDE226, PDS, PHYTOENE DESATURASE, PIGMENT DEFECTIVE 226, phytoene desaturase 3)
启动子:Arabidopsis U6 polymerase III promoter
克隆方法:限制性内切酶
5'克隆位点:SacI (not destroyed)
3'克隆位点:SmaI (destroyed during cloning)
5'测序引物:5’ TGGAATTGTGAGCGGATA 3’
3'测序引物:5’ ATTAAGTTGGGTAACGCC 3'
质粒图谱: