质粒名称:pTriEx-mCherry-PA-Rac1
质粒编号:22027
质粒备注:Expression of photoactivatable Rac1
载体名称:pTriEx
载体大小:6000
载体类型:Mammalian Expression, Bacterial Expression, Insect Expression
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:PA-Rac1
基因别名:LOV2-Rac1
物种来源:H. sapiens (human); Avena sativa (oat)
基因突变:LOV(Leu404-Leu546), Rac1 starts at Ile4 and contains mutations Q61L, E91H and N92H
插入大小:1100
基因名称:RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
启动子:CMV, p10
标签/融合蛋白:· 6xHis (N terminal on backbone)
· mCherry (N terminal on backbone)
克隆方法:限制性内切酶
5'克隆位点:Bam HI (not destroyed)
3'克隆位点:HindIII (not destroyed)
5'测序引物:pTriExUP
质粒图谱: