质粒名称:pT7-gRNA
质粒编号:46759
质粒备注:gRNA cloning vector for in vitro transcription of target gRNA
载体名称:pUC19
载体大小:2400
完整质粒大小:2540
载体类型:CRISPR ; zebrafish expression
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Top10
高拷贝/低拷贝:高拷贝
插入基因:gRNA core
物种来源:Synthetic
插入大小:76
启动子:T7
克隆方法:限制性内切酶
5'克隆位点:HindIII (destroyed during cloning)
3'克隆位点:BamHI (not destroyed)
5'测序引物:M13 R
3'测序引物:M13 F
质粒图谱: