质粒名称:pRK793
质粒编号:8827
载体名称:pMal-C2
载体大小:6700
载体类型:原核表达
原核抗性:Chloramphenicol and Ampicillin, 25 & 100 μg/mL
培养温度:37℃
克隆菌株:BL21(DE3)-RIL
高拷贝/低拷贝:低拷贝
插入基因:TEV protease, S219V mutant
基因别名:tobacco etch virus protease
基因突变:S219V mutation (improves stability)
插入大小:750
标签/融合蛋白:· His (N terminal on insert)
· polyarginine (C terminal on insert)
· MBP (with TEV) (N terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:SacI (not destroyed)
3'克隆位点:BamHI (not destroyed)
3'测序引物:M13-F20
质粒图谱: