质粒名称:pRH2502
质粒编号:84379
质粒备注:Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter
载体名称:pTC-0X-1L
载体大小:4640
完整质粒大小:8779
载体类型:Bacterial Expression, CRISPR
原核抗性:卡那霉素,50μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:低拷贝
插入基因:dcas9
基因别名:cas9 D10A and H840A
物种来源:Streptococcus pyogenes
基因突变:Aspartate at position 10 changed to Alanine, Histidine at position 840 changed to Alanine
插入大小:4139
启动子:uv15tetO
克隆方法:限制性内切酶
5'克隆位点:NdeI (not destroyed)
3'克隆位点:PacI (not destroyed)
质粒图谱: