质粒名称:pMJ915
质粒编号:69090
质粒备注:Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS
载体名称:pML-2CT
载体大小:5950
完整质粒大小:10094
载体类型:原核表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:cas9
物种来源:Streptococcus pyogenes
插入大小:5358
启动子:T7
标签/融合蛋白:· MBP (N terminal on backbone)
· hexa His (N terminal on backbone)
· two SV40 NLS (C terminal on insert)
克隆方法:Ligation Independent Cloning
5'测序引物:T7 promoter
3'测序引物:T7 terminator
质粒图谱: