质粒名称:pLinker-AID-3xHA-DHFR-LoxP
质粒编号:86669
质粒备注:for generation of AID strains using CRISPR tagging technology
载体名称:pUC19
载体类型:Expression in Toxoplasma gondii
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:Linker-AID-3xHA-HXGPRT 3'UTR
物种来源:Toxoplasma gondii
插入大小:1263
克隆方法:限制性内切酶
5'克隆位点:NheI (not destroyed)
3'克隆位点:PxpXI (not destroyed)
5'测序引物:GGGCCCgctagcAAGGGCTCGGGCTCGACCCAGCTGATGGGCAGTGTCGAGCT
3'测序引物:GACCCTCGAGTAGAACTAGTGGATCCGAGC
质粒图谱: