质粒名称:pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase
质粒编号:119816
质粒备注:3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells
载体名称:pLentipuro3/TO/V5-DEST
载体大小:8116
完整质粒大小:10489
载体类型:Mammalian Expression, Lentiviral, Luciferase
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:Luciferase
基因别名:Firefly luciferase
物种来源:Photinus pyralis
基因突变:Stop codon removed in the frame of the V5 frame vector
插入大小:2373
启动子:CMV/TO
标签/融合蛋白:· EGFP (N terminal on insert)
· V5 (C terminal on insert)
克隆方法:Gateway Cloning
5'测序引物:CMV Forward
3'测序引物:V5 Reverse
质粒图谱: