质粒名称:pGfa2-nLac
质粒编号:53126
质粒备注:Expression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.
载体名称:pUC18
载体类型:Mammalian Expression, Mouse Targeting
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:Gfa2
基因别名:glial fibrillary acidic protein
基因别名:GFAP
物种来源:H. sapiens (human)
基因突变:contains -2163 to +47 (the human gfa2 segment), with the initiating ATG mutated to TTG
基因名称:GFAP (a.k.a. ALXDRD)
启动子:human GFAP
标签/融合蛋白:· NLS (C terminal on insert)
· LacZ (C terminal on insert)
· mP1 (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:BglII (not destroyed)
3'克隆位点:BglII (not destroyed)
5'测序引物:MB-351 CATCGCCAGTCTAGCCCACTCCT
3'测序引物:MB-352 GACGTTGTAAAACGACGGCCAGT
质粒图谱: