质粒名称:pFGC-pcoCas9
质粒编号:52256
质粒备注:A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA
载体名称:pFGC-RCS
载体类型:CRISPR ; Plant expression
筛选标记:Basta
原核抗性:卡那霉素,50μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:35SPPDK:pcoCas9:NOS cassette
基因别名:Cas9
基因别名:plant codon optimized Cas9
物种来源:Synthetic
启动子:constitutive 35SPPDK promoter
克隆方法:限制性内切酶
5'克隆位点:StuI (not destroyed)
3'克隆位点:StuI (not destroyed)
5'测序引物:Sequencing primer for multiple cloning sites (from somewhere close to the EcoRI site): 5’ AATAAAAACTGACTCGGA 3’
3'测序引物:Sequencing primer for 35SPPDK:pcoCas9:NOS (from somewhere close to the 35SPPDK promoter): 5’ AGTTGGGTAACGCCAGGG 3’
质粒图谱: