质粒名称:pComb3XTT
质粒编号:63891
质粒备注:Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)
载体名称:pComb3X
载体大小:3300
完整质粒大小:4700
载体类型:Bacterial Expression ; phage display
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:XL1 Blue
高拷贝/低拷贝:高拷贝
插入基因:TT Fab
基因别名:human Fab to tetanus toxin
物种来源:H. sapiens (human)
插入大小:1400
启动子:lacZ
标签/融合蛋白:· 6xHis (C terminal on backbone)
· HA tag (C terminal on backbone)
· geneIII (C terminal on backbone)
克隆方法:限制性内切酶
5'克隆位点:SfiI (not destroyed)
3'克隆位点:SfiI (not destroyed)
5'测序引物:AAG ACA GCT ATC GCG ATT GCA G
3'测序引物:GCC CCC TTA TTA GCG TTT GCC ATC
质粒图谱: