质粒名称:pComb3XSS
质粒编号:63890
质粒备注:3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment
载体名称:pComb3H
载体大小:3300
完整质粒大小:4900
载体类型:Bacterial Expression ; phage display
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:XL1 Blue
高拷贝/低拷贝:高拷贝
插入基因:SS Stuffer
基因别名:SfiI Stuffer
物种来源:Synthetic
插入大小:1600
启动子:lacZ
标签/融合蛋白:· 6x His (C terminal on backbone)
· HA tag (C terminal on backbone)
· geneIII (C terminal on backbone)
克隆方法:限制性内切酶
5'克隆位点:SfiI (not destroyed)
3'克隆位点:SfiI (not destroyed)
5'测序引物:5'-AAG ACA GCT ATC GCG ATT GCA G-3'
3'测序引物:5'-GCC CCC TTA TTA GCG TTT GCC ATC-3'
质粒图谱: