质粒名称:pCMV-PEmax-P2A-hMLH1dn
质粒编号:174828
质粒备注:Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)
载体名称:pCMV
载体类型:哺乳动物表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:PEmax-P2A-hMLH1dn
基因别名:SV40_bpNLS-SpCas9(R221K,N394K,H840A)-SGGSx2-SV40_bpNLS-SGGSx2-MMLV_RT(D200N, T306K, W313F, T330P, L603W)-SV40_bpNLS-cMyc_NLS
基因别名:homo sapiens MLH1 del754-756 (codon optimized)
物种来源:H. sapiens (human), Synthetic; SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus
基因突变:Detailed in manuscript; deletion of residues 754-756 in MLH1
插入大小:8715
启动子:CMV
标签/融合蛋白:· SV40 bpNLS (N terminal on insert)
· SV40 bpNLS (C terminal on insert)
· c-Myc NLS (C terminal on insert)
克隆方法:Ligation Independent Cloning
5'测序引物:T7-forward
3'测序引物:BGH-reverse
质粒图谱: