质粒名称:pCAS1yl
质粒编号:73226
质粒备注:Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells
载体名称:pMCSCen1
载体类型:Bacterial Expression, CRISPR ; in Y. lipolytica
筛选标记:LEU2
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:Cas9
物种来源:S. pyogenes MGAS5005
启动子:unknown
克隆方法:限制性内切酶
5'克隆位点:MluI (not destroyed)
3'克隆位点:SalI (not destroyed)
5'测序引物:M13R
3'测序引物:M13F20
质粒图谱: