质粒名称:pCas12f1
质粒编号:171182
质粒备注:Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1
载体名称:pCas
载体大小:8576
完整质粒大小:12545
载体类型:Bacterial Expression, CRISPR
原核抗性:卡那霉素,50μg/mL
培养温度:30℃
克隆菌株:DH5alpha
高拷贝/低拷贝:低拷贝
插入基因:Cas12f1
基因别名:Cas14a1
物种来源:Synthetic
插入大小:1587
启动子:cas9 promoter from Streptococcus pyogenes
克隆方法:限制性内切酶
5'克隆位点:SalI/XhoI (destroyed during cloning)
3'克隆位点:KpnI (not destroyed)
5'测序引物:GTATTGGGTAATATTTTTTGAAGAGATATTTTG
3'测序引物:CATTCAAATATGTATCCGCTCATG
质粒图谱: