质粒名称:pCas
质粒编号:62225
质粒备注:Constitutive expression of cas9 and inducible expression of lambda RED and sgR
载体名称:pKD46
载体大小:6300
载体类型:Bacterial Expression, CRISPR ; Lambda Red recombinase
原核抗性:卡那霉素,50μg/mL
培养温度:30℃
克隆菌株:DH5alpha
高拷贝/低拷贝:低拷贝
插入基因:cas9
物种来源:S. pyogenes MGAS5005
插入大小:4500
启动子:native cas9 promoter
克隆方法:限制性内切酶
5'克隆位点:unknown (unknown if destroyed)
3'克隆位点:unknown (unknown if destroyed)
5'测序引物:pENTR1A-3 (GTAACATCAGAGATTTTGAGACAC)
3'测序引物:AraC-F-New (GATGTAGCCGTCAAGTTGTCA)
质粒图谱: