质粒名称:pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090
质粒编号:103871
质粒备注:dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
载体名称:pcDNA3.1
载体大小:5428
完整质粒大小:9525
载体类型:哺乳动物表达,CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:PspCas13b
物种来源:Prevotella sp. P5-125
基因突变:Changed histidne 133 to alanine and histidine 1058 to alanine to remove RNAse activity. Lacks residues 984-1090.
插入大小:2949
质粒图谱: