质粒名称:pBad-EBFP2
质粒编号:14891
质粒备注:Bacterial expression of EBFP2 blue fluorescent protein
载体名称:pBad/His B
载体大小:4100
载体类型:原核表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:低拷贝
插入基因:EBFP2
基因别名:EBFP2 blue fluorescent protein
物种来源:evolved fluorescent protein
基因突变:The fluorescent protein was inserted between XhoI and EcoR1 of pBad/His B. There is an extra 'C' after XhoI to avoid the frameshift.
插入大小:720
克隆方法:限制性内切酶
5'克隆位点:XhoI (not destroyed)
3'克隆位点:EcoRI (not destroyed)
5'测序引物:ATGCCATAGCATTTTTATCC
3'测序引物:GATTTAATCTGTATCAGG
质粒图谱: