质粒名称:NL4-3 mCherry Luciferase
质粒编号:44965
质粒备注:HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.
载体名称:pNLENG1
完整质粒大小:12424
载体类型:慢病毒
筛选标记:mCherry, Luciferase
原核抗性:氨苄青霉素,100μg/mL
培养温度:30℃
克隆菌株:Stbl3
高拷贝/低拷贝:未知
插入基因:mCherry, Luciferase, IRES
基因别名:HIV 1 reporter virus
物种来源:Lentivirus, Human specific
基因突变:S34C mutation in Nef
启动子:EF-1 alpha– driven mCherry:Puromycin
标签/融合蛋白:· mCherry with D212G (C terminal on insert)
· Luciferase, IRES (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:BamHI (not destroyed)
3'克隆位点:XhoI (destroyed during cloning)
5'测序引物:from pNLENG1 5'
3'测序引物:from pNLENG1 3'
质粒图谱: