质粒名称:MSP1673
质粒编号:65769
质粒备注:Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA
载体名称:pACYCDuet-1
载体类型:Bacterial Expression, CRISPR
原核抗性:氯霉素25μg/mL
培养温度:37℃
克隆菌株:XL1 Blue
高拷贝/低拷贝:低拷贝
插入基因:mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
启动子:T7 (x2)
克隆方法:Gibson Cloning
5'测序引物:ACYC-DuetUP1
3'测序引物:oBK390 (5'-GCAAATTCGACCCGGTCGTCG-3')
质粒图谱: