质粒名称:MCS-BioID2-HA
质粒编号:74224
质粒备注:(Empty Backbone) To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
载体名称:pcDNA 3.1
载体大小:6113
载体类型:哺乳动物表达
筛选标记:新霉素(G418)
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
启动子:CMV
标签/融合蛋白:· humanized A. aeolicus BioID2 with R40G (C terminal on backbone)
· HA (C terminal on backbone)
克隆方法:限制性内切酶
5'测序引物:CMV-F
3'测序引物:BGH-rev
质粒图谱: