质粒名称:Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
质粒编号:118155
质粒备注:Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
载体名称:lentiCRISPRv2
载体类型:哺乳动物表达,慢病毒,CRISPR
筛选标记:Blasticidin
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Stbl3
高拷贝/低拷贝:高拷贝
插入基因:KRAB-dCas9-P2A-BlastR
物种来源:H. sapiens (human)
插入大小:4908
启动子:hPGK and U6
克隆方法:限制性内切酶
5'克隆位点:Unknown (not destroyed)
3'克隆位点:Unknown (not destroyed)
3'测序引物:CTTGCTGGGCACCTTGTACT
质粒图谱: