质粒名称:GLP1R-Tango
质粒编号:66295
质粒备注:Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
载体名称:empty Tango
载体大小:6632
载体类型:哺乳动物表达
筛选标记:新霉素(G418)
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:GLP1R
物种来源:H. sapiens (human)
插入大小:1389
基因名称:GLP1R (a.k.a. GLP-1, GLP-1-R, GLP-1R)
启动子:CMV
克隆方法:限制性内切酶
5'克隆位点:NotI (not destroyed)
3'克隆位点:XhoI (unknown if destroyed)
5'测序引物:CMV-F
3'测序引物:Tet-R
质粒图谱: