质粒名称:CTGF-bio-His
质粒编号:52028
质粒备注:Expresses full-length Connective tissue growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
载体名称:pTT3
载体大小:6618
完整质粒大小:7685
载体类型:哺乳动物表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Top10
高拷贝/低拷贝:高拷贝
插入基因:CTGF
基因别名:Connective tissue growth factor precursor
物种来源:H. sapiens (human)
插入大小:1067
基因名称:CCN2 (a.k.a. CTGF, HCS24, IBP-8, IGFBP8, NOV2)
启动子:CMV
标签/融合蛋白:· rat CD4 d3+4 (C terminal on backbone)
· enzymatic biotinylation sequence (C terminal on backbone)
· His tag (C terminal on backbone)
克隆方法:限制性内切酶
5'克隆位点:NotI (not destroyed)
3'克隆位点:AscI (not destroyed)
5'测序引物:CMV-fwd
质粒图谱: