质粒名称:Cas9-NAT
质粒编号:64329
质粒备注:Cas9 expression cassette carried by a yeast single-copy episome plasmid
载体名称:pRS414-TEF1p-Cas9-CYC1t
载体大小:9232
完整质粒大小:10446
载体类型:Yeast Expression, CRISPR
筛选标记:Nourseothricin(clonNat)
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:低拷贝
插入基因:Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
插入大小:1206
克隆方法:限制性内切酶
5'克隆位点:SnaBI (not destroyed)
3'克隆位点:MfeI (not destroyed)
5'测序引物:tctagagcggccgcTACGTAgatctgtttagcttgccttg
3'测序引物:tctagagcggccgcCAATTGcgttttcgacactggatggc
质粒图谱: