质粒名称:BPK1520
质粒编号:65777
质粒备注:Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA
载体名称:MLM3636
载体类型:哺乳动物表达,CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:XL1 Blue
高拷贝/低拷贝:高拷贝
插入基因:SpCas9 gRNA backbone, without spacer sequence
物种来源:Synthetic
启动子:U6
克隆方法:Gibson Cloning
5'测序引物:OS280 (5'-CAGGGTTATTGTCTCATGAGCGG-3')
质粒图谱: