产品介绍 目录号
Serial | SCSP-5057 | 标识符
Identifier | CSTR:19375.09.3101HUMSCSP5057 | 描述
Description | 该细胞系来自11岁白人女性的组织。该患者医疗使用过 RTG, methotrexate, adriamycin, vincristine, cytoxan, and aramycin-C。该细胞在免疫抑制小鼠中不致瘤，但在半固体培养基中形成集落。在我库通过支原体检测为阴性。冻存时为P35代。经本库STR检测无误。STR鉴定结果为：Amelogenin: X；CSF1PO: 10；D13S317: 12,13；D16S539: 12,13；D5S818: 12；D7S820: 8,10；THO1: 6,9；TPOX: 8；vWA: 18 | 动物种别
Organism | 人 | 性别
Gender | 女性，11岁 | 组织来源
Tissue and Cell Type | 骨 | 形态
Morphology | 上皮样，贴壁生长 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | 人骨肉瘤细胞Saos-2完全培养液配方(100 ml)：
McCoy's 5a Medium (Invitrogen, 12330-031) 85 ml
FBS (Gibco) 15 ml
备注：Saos-2细胞系通常没有很多碎片。但是，它始终都会有一些漂浮物。培养基中的小黑点可能是碎片，这可能因为没有达到***培养条件。我们建议仔细核对培养基配方和培养条件。请在细胞融合之前进行传代培养，并定期更换培养基。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Banerjee C, et al. An AML-1 consensus sequence binds an osteoblast-specific complex and transcriptionally activates the osteoclacin gene. Proc. Natl. Acad. Sci. USA 93: 4968-4973, 1996. PubMed: 8643513Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034Takeuchi Y, et al. Relationship between actions of transforming growth factor (TGF)-beta and cell surface expression of its receptors in clonal osteoblastic cells. J. Cell. Physiol. 162: 315-321, 1995. PubMed: 7860639Zhang W, et al. EGF-mediated phosphorylation of extracellular signal-regulated kinases in osteoblastic cells. J. Cell. Physiol. 162: 348-358, 1995. PubMed: 7860643Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212Schar BK, et al. Simultaneous detection of all four alkaline phosphatase isoenzymes in human germ cell tumors using reverse transcription-PCR. Cancer Res. 57: 3841-3846, 1997. PubMed: 9288797Morris GF, et al. Transcriptional activation of the human proliferating-cell nuclear antigen promoter by p53. Proc. Natl. Acad. Sci. USA 93: 895-899, 1996. PubMed: 8570655Werner H, et al. Wild-type and mutant p53 differentially regulate transcription of the insulin-like growth factor I receptor gene. Proc. Natl. Acad. Sci. USA 93: 8318-8323, 1996. PubMed: 8710868Wang X, et al. Variabilin, a novel RGD-contining antagonist of glycoprotein IIb-IIIa and platelet aggregation inhibitor from the gard tick Dermacentor variabilis. J. Biol. Chem. 271: 17785-17790, 1996. PubMed: 8663513Karnieli E, et al. The IGF-1 receptor gene promoter is a molecular target for the Ewing's Sarcoma=Wilms' Tumor 1 fusion protein. J. Biol. Chem. 271: 19304-19309, 1996. PubMed: 8702614 |
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