产品介绍 目录号
Serial | SCSP-305 | 标识符
Identifier | CSTR:19375.09.3101HUMSCSP305 | 描述
Description | BG01V是一株具有异常核型的人类胚胎干细胞系。尽管有异常的核型(49, XXY, +12, +17)，但当在小鼠胚胎饲养层(MEFs)上生长时，这些克隆状仍显示出均匀的形态，可预测生长速率，并且在培养中易于维持。据报道，细胞的多能性标记和碱性磷酸酶活性呈阳性。每个批次均通过本库支原体检测，结果为阴性。我库使用的饲养层为CF-1 MEF ( SCSP-105C 、 SCSP-105R 或 SCSP-110R )
经本库STR检测结果无误。STR结果如下：D5S818:10,12；D13S317: 11,12；D7S820: 10,11；D16S539: 9,11；vWA: 16,17；THO1: 7,9.3；Amelogenin: X,Y；TPOX:8,8；CSF1PO: 10,10 | 动物种别
Organism | 人 | 性别
Gender | 性染色体XXY | 组织来源
Tissue and Cell Type | 内细胞团 | 形态
Morphology | 球形克隆，贴壁生长 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | hES完全培养液配方：
DMEM/F12(Invitrogen, 11330)466 ml
Knockout Serum Replacement(Invitrogen, 10828)120 ml
Non-essential Amino Acids, 100 (Invitrogen, 11140) 6 ml
Glutamax(invitrogen 35050) 6 ml
55mM 2-mercaptoethanol(Invitrogen, 21985) 1.5 ml
bFGF (Invitrogen, 13256)
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Embryonic Stem Cell Lines Derived from Human Blastocysts, James A. Thomson, et al. Science 282, 1145(1998)Zeng X, et al. Properties of pluripotent human embryonic stem cells BG01 and BG02. Stem Cells 22: 292-312, 2004. PubMed:15153607Brimble S, et al. Karyotypic stability, genotyping, differentiation, feeder free maintenance and gene expression sampling in three human embryonic stem cell lines derived prior to August 9th 2001. Stem Cells Dev. 13(6): 585-597, 2004. PubMed: 15684826Mitalipova M, Calhoun J, Shin S, Wininger D, Schulz T, Noggle S, Venable A, Lyons I, Robins A, Stice S. (2003). Human embryonic stem cell lines derived from discarded embryos. Stem Cells, 21(5), 521-6. PubMed: 12968106Schulz TC, Noggle S, Palmarini G, Weiler D, Lyons I, Pensa K, Meedeniya A, Davidson B, Lambert N, Condie B (2004). Differentiation of Human Embryonic Stem Cells to Dopaminergic Neurons in Serum Free Suspension Culture. Stem Cells, 22(7), PMID: 15579641Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988 |
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