细胞培养基 >> 细胞株
786-O [786-0]人肾细胞腺癌细胞(新引进)
产品介绍 目录号 Serial | SCSP-5059 | 标识符 Identifier | CSTR:19375.09.3101HUMSCSP5059 | 描述 Description | 该细胞系来自58岁高加索男性原发性透明细胞腺癌。细胞同时具有微绒毛和桥粒,可在软琼脂中生长。这些细胞产生一种类似于肽的PTH,与乳腺和肺肿瘤产生的肽相同。该肽具有与PTH类似的N末端序列,具有PTH样活性,分子量为6000道尔顿。每个批次均通过本库支原体检测,结果为阴性。经本库STR检测无误。STR鉴定结果为:Amelogenin: X,Y;CSF1PO: 10;D13S317: 8;D16S539: 12;D5S818: 9;D7S820: 11,12;THO1: 6,9.3;TPOX: 8,11;vWA: 15,17。 | 动物种别 Organism | 人 | 性别 Gender | 男性 | 组织来源 Tissue and Cell Type | 肾脏 | 形态 Morphology | 上皮样,贴壁生长 | 培养基和添加剂 Complete Growth Medium and Culture Conditions | 人肾细胞腺癌细胞786-O [786-0]完全培养液配方(100 ml): RPMI 1640 Medium (Invitrogen, 11875-093) 88 ml FBS (Gibco)10 ml Glutamax (Invitrogen 35050061) 1 ml Sodium Pyruvate 100 mM Solution (Invitrogen 11360070) 1 ml 参考传代比例:1:3 参考传代周期:3-4天 参考换液频率:每周2次 冻存液配方:完全培养液95%,DMSO 5% 培养温度:37摄氏度。 | 供应限制 Permits and Restrictions | A。仅供研究之用。 | REFERENCE | Williams RD, et al. In vitro cultivation of human renal cell cancer. I. Establishment of cells in culture. In Vitro 12: 623-627, 1976. PubMed: 1010528 Williams RD, et al. In vitro cultivation of human renal cell cancer. II. Characterization of cell lines. In Vitro 14: 779-786, 1978. PubMed: 721102 Thiede MA, et al. Human renal carcinoma expresses two messages encoding a parathyroid hormone-like peptide: evidence for the alternative splicing of a single- copy gene. Proc. Natl. Acad. Sci. USA 85: 4605-4609, 1988. PubMed: 3290897 MacKay K, et al. Glomerular epithelial, mesangial, and endothelial cell lines from transgenic mice. Kidney Int. 33: 677-684, 1988. PubMed: 2835539 |
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